ISSN (Online): 2456-6268
Journal of Fisheries and Life Sciences

Journal of Fisheries and Life Sciences

2020, Vol. 5 Issue 2, Part DECEMBER
Molecular detection of a soil nematode (Panagrellus redivivus) in artificial culture media
Author(s): Mohammed Mostafa, S. M. Nazmus Salehin, Al-Imran, Abul Farah Md. Hasanuzzaman, Sharmin Aktar and Md. Shahin Parvez
Abstract: Nematodes are thought to be troublesome organisms to identify due to few apparent taxonomic characteristics with overlapping morphometric and morphological similarity among the species. Molecular based techniques are more sensitive and reliable than microscopic morphological study to identify the species and subspecies avoiding similar ambiguity. Therefore, this study was undertaken to apply PCR analysis in identifying a soil nematode, Panagrellus redivivus cultured in potato, ceral and oatmeal media so as to show the feasibility of those artificial media as suitable for P. redivivus production. To do so, the starter of P. redivivus was cultured in those artificial media prepared in association with baker’s yeast. The P. redivivus grown in each media were heated and centrifuged to isolate the DNA which were finally amplified with Pan28s (F/R) and Pan28s NES(F/R) primers retrieved from 28s ribosomal RNA gene of the species. A fragment of 1131-bp for Pan28s (F/R) primer in case of first step PCR and a 1050-bp band for Pan28s NES (F/R) in case of 2nd step PCR (nested) confirmed the species. By identifying the species at molecular level in those media, the study concluded that the three media were suitable for artificial culture of P. redivivus species to be used as a potential alternative live feed in prawn hatcheries in Bangladesh
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